Why Fluorometry is a More Effective Technique than Spectroscopy

Why is Fluorometry a better technique that Spectroscopy

Increased sensitivity and more importantly can accurately measure DNA concentration of a structurally intact double stranded DNA molecule

Fluorometry is a better technique than spectroscopy for several reasons:

1. Higher sensitivity: Fluorometry is a highly sensitive technique as it measures the intensity of fluorescence emitted from a sample. The fluorescence signal is much higher than the absorbance signal obtained in spectroscopy, which improves the detection limits of fluorometry.

2. Better selectivity: Fluorometry is more selective than spectroscopy as it can differentiate between very similar molecules based on their emission spectra. This is due to each molecule emitting a unique fluorescence profile that can be captured through fluorometry.

3. Lower interference: In spectroscopy, interference from other compounds in the sample can result in incorrect measurements. Fluorometry is less prone to interference as it only measures the fluorescence signal, which is less likely to be affected by other substances.

4. Broader application: Fluorometry can be applied to a wider range of samples, including biological molecules, pollutants, and chemical compounds, making it a versatile and useful technique in various fields of research.

5. Non-destructive method: Fluorometry is a non-destructive method as the sample is not consumed during the analysis. The fluorescence measurement can be taken multiple times on the same sample, which is not possible in spectroscopy, where the sample is consumed after each measurement.

6. Increased accuracy: Fluorometry is a more accurate technique than spectroscopy as it has a lower coefficient of variation (CV). This results in more reliable and precise measurements, which is essential for many scientific applications.

Therefore, fluorometry is a better technique than spectroscopy due to its higher sensitivity, better selectivity, lower interference, broader application, non-destructive nature, and increased accuracy.

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