Science | Senioritis

Mohamed Abbas
August 27, 2023
Biochemistry

Resolving Protein Sizes with SDS-PAGE

What is meant by 4 –12% or 8% SDS-PAGE? SDS-PAGE stands for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis. It is a widely used technique in biochemistry and...

Mohamed Abbas
August 27, 2023
Biochemistry

Determining the Thickness of a Membrane in a Transmembrane Protein

What is the thickness of the membrane if only alpha helixes are embedded of a transmembrane protien? The thickness of the membrane when only α-helices are embedded...

Mohamed Abbas
August 27, 2023
Biochemistry

Kir2.1 Inward-Rectifying Potassium Channel

Confusion regarding the Kir2.1 inward-rectifying potassium channel The Kir2.1 inward-rectifying potassium channel is a specific type of protein that plays a crucial role in allowing the movement...

Mohamed Abbas
August 27, 2023
Biochemistry

Powerful Technique for Molecular Manipulations & DNA Sequencing

How does PCR mutagenesis add restriction site near the gene of interest? PCR mutagenesis is a powerful technique used to introduce specific mutations into a DNA sequence....

Mohamed Abbas
August 27, 2023
Biochemistry

Nomenclature of Substrates for DNA Synthesis

Nomenclature of substrates for DNA synthesis The nomenclature of substrates for DNA synthesis refers to the systematic naming convention used to describe the various components involved in...

Mohamed Abbas
August 27, 2023
Biochemistry

Optimizing Co-Immunoprecipitation

How to incerase the efficiency of co-immunoprecipitation? To increase the efficiency of co-immunoprecipitation, several key factors should be considered and optimized. Co-immunoprecipitation is a widely used technique...

Mohamed Abbas
August 27, 2023
Biochemistry

The Anomalous NAD/FAD Redox Reaction in Pyruvate Dehydrogenase

Pyruvate dehydrogenase: Apparently anomalous NAD/FAD redox reaction Pyruvate dehydrogenase is an enzyme complex found in the mitochondria of cells. Its main function is to convert pyruvate, a...

Mohamed Abbas
August 27, 2023
Biochemistry

Cloning a Gene with His Tag & TEV Cleavage Site

How can I clone a gene into a plasmid vector with an N-terminal his tag and TEV cleavage site between the tag and the start of the...