Science | Senioritis

Mohamed Abbas
June 20, 2023
Pathology

6 Strategies to Avoid PCR Contamination in Your Laboratory

Best way to avoid PCR contamination Separate Pre-PCR (clean area) and post PCR (dirty area) PCR contamination can be a major problem in many laboratories. Here are...

Mohamed Abbas
June 20, 2023
Pathology

Significance of A260/A280 Ratio and Protein Contamination in RNA Samples

A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA Protein contamination . A260/280 = 1.3

Mohamed Abbas
June 20, 2023
Pathology

Why Fluorometry is a More Effective Technique than Spectroscopy

Why is Fluorometry a better technique that Spectroscopy Increased sensitivity and more importantly can accurately measure DNA concentration of a structurally intact double stranded DNA molecule Fluorometry...

Mohamed Abbas
June 20, 2023
Pathology

Understanding Linkage Disequilibrium: Implications for Genetic Studies and Disease Mapping

Linkage Disequilibrium LD is the non random association of alleles at 2 or more loci in a general population. When alleles are in LD, haplotypes do not...

Mohamed Abbas
June 20, 2023
Pathology

Understanding Specificity in Medical Testing: Importance and Evaluation Measures

Specificity Specificity = TN / (TN + FP) In biology and medicine, specificity refers to the ability of a test, measurement or diagnostic tool to accurately identify...

Mohamed Abbas
June 20, 2023
Pathology

Understanding Limit of Detection (LOD) in Analytical Methods for Science Related Fields

Limit of Detection (LOD) lowest concentration of analyte that can be detected Limit of Detection (LOD) refers to the lowest amount of a substance or analyte that...

Mohamed Abbas
June 20, 2023
Pathology

How to Convert an mRNA Sequence into DNA: A Step-by-Step Guide with Examples

mRNA Sequence provided – Convert it into DNA (Template/Anti-sense Strand) [Replace U with T] To convert an mRNA sequence into DNA, we need to follow the rules...

Mohamed Abbas
June 20, 2023
Pathology

Understanding the Essential Steps Involved in HIV Sample Preparation Workflow for Accurate Detection and Quantification

Correct order of HIV Sample Preparation Workflow Plasma PreparationRNA ExtractionRNA Amplification/Modification Procedure The correct order of HIV sample preparation workflow includes the following steps: 1. Sample collection:...