what does UV spectroscopy examine?
transitions b/t vibration energy levels
UV spectroscopy is a type of spectroscopy that examines the absorption or transmission of ultraviolet (UV) light by a sample. This technique is used to study the electronic structure of molecules, particularly the transition of electrons from ground state to excited state.
More specifically, UV spectroscopy examines the chemical bonds and functional groups that absorb radiation in the UV region (typically 200-400 nm). This includes groups such as pi-bonds and chromophores, which are responsible for the color of compounds. By measuring the amount of UV light absorbed at different wavelengths, information about the identity and concentration of a sample can be obtained.
UV spectroscopy is often used in biochemistry, molecular biology, and pharmaceuticals to study the purity and concentration of proteins, DNA, and drugs. It is also used in environmental and forensic analysis to identify and quantify pollutants and other substances.
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