Exploring the Significance of Amide II Band in IR Spectroscopy: Insights into Protein and Peptide Structure

Amide 2

-CONH2, -CONHR, -CONR2

Amide 2 is a region of the infrared (IR) spectrum where amide bonds in proteins and peptides absorb light in a characteristic way. The amide bond is a covalent bond between the alpha-carboxyl group of one amino acid and the alpha-amino group of another amino acid in a protein or peptide chain.

The Amide II band lies in the range of 1480-1575 cm^-1 and typically arises due to C-N stretching mode along with N-H bending vibrations. The absorption at Amide II region in infrared spectroscopy is used to study the conformation changes in proteins and peptides.

The intensity and position of Amide II absorbance can provide significant information on the secondary structure of proteins/peptides such as alpha helices, beta sheets, etc. The shape of the Amide II band can also be used as an indicator of protein hydration, protein denaturation, and protein-protein or protein-ligand interactions.

In summary, the Amide II band is an important structural characteristic of proteins and peptides, and its study can provide insights into the conformation and function of these molecules.

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