What is the purpose of Periode acid-Sciff (PAS)?
It stains the hyphae of molds and some yeasts
The Periodic acid-Schiff (PAS) stain is a histological staining technique that is used to detect the presence of carbohydrates and glycoproteins in a tissue. This staining technique is commonly used in pathology and histology labs to diagnose and classify various diseases involving carbohydrate-rich structures such as mucopolysaccharidoses, glycogen storage diseases, and some epithelial tumors.
The purpose of PAS staining is to identify and visualize the presence of these carbohydrate-rich structures in tissue sections. In the PAS staining process, periodic acid is used to oxidize the carbohydrates present in the tissue, which then reacts with the Schiff reagent to produce a colored product that can be visualized microscopically. The stained carbohydrate-rich structures appear red or magenta, providing a contrast against the blue or green background of the tissue section.
Overall, the PAS staining technique can aid in diagnosis and classification of diseases involving carbohydrate-rich structures, providing valuable information for clinicians and researchers.
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