Gram-positive bacteria stain ______.
Blue
Gram-positive bacteria stain purple when subjected to the Gram staining technique. This is because the thick peptidoglycan layer in the bacterial cell wall retains crystal violet stain after the initial step of staining. Gram-positive bacteria have a thicker layer of peptidoglycan than Gram-negative bacteria, which allows them to retain the stain more effectively. After the application of a mordant and a decolorizing agent, the purple color of the Gram-positive bacterial cells remains intact, while the Gram-negative bacterial cells lose their stain and become colorless.
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