In the Gram stain procedure _______ is the mordant.
Gram’s Iodine
In the Gram stain procedure, the mordant used is Gram’s iodine solution. The mordant is used to fix the primary stain (crystal violet) onto the bacterial cell wall and enhance the contrast of the stained specimen. Gram’s iodine forms complexes with crystal violet molecules and makes the stain insoluble in water. This results in the retention of the purple color of the bacteria after washing with a decolorizing solution, which allows for the differentiation of Gram-positive and Gram-negative bacteria.
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