In a protein, the most conformationally restricted amino acid is ______; the least conformationally restricted is ______.
Pro, Gly
The most conformationally restricted amino acid in a protein is proline, due to its unique cyclic structure that creates a rigid kink in the peptide backbone. Proline’s cyclic structure also makes it difficult to incorporate into protein structures and can affect the protein’s folding stability.
On the other hand, the least conformationally restricted amino acid is glycine, due to its small and flexible side chain that allows it to adopt a wide range of conformations. Glycine’s flexibility allows it to serve as a “hinge” in protein structures and can affect the protein’s overall flexibility.
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