By increasing the percentage of acrylamide in an SDS-PAGE gel, one will observe what following change in the molecular weights of the observed proteins?
increased range of molecular weights observed
Increasing the percentage of acrylamide in an SDS-PAGE gel will result in increased resolution of lower molecular weight proteins and decreased resolution of higher molecular weight proteins. This is because higher percentages of acrylamide create a tighter mesh size, which slows the migration of larger proteins through the gel matrix. Conversely, smaller proteins will be able to navigate through the smaller pores created by higher acrylamide content and will appear well-separated on the gel. Therefore, by increasing the percentage of acrylamide in an SDS-PAGE gel, the observed proteins will appear more resolved, with a clearer, sharper banding pattern for proteins with molecular weights in the lower range.
More Answers:
Protein Sequencing Simplified: The Significance of Edman Degradation TechniqueExploring the Titratable Functional Groups in Peptide MDFGRKCDE: A Science Analysis
Analyzing SARS-CoV-2 Spike Protein Structural Properties: G76V Mutation More Likely to Occur Than V180G
Error 403 The request cannot be completed because you have exceeded your quota. : quotaExceeded