What technique can be used for studying the gene for cystic fibrosis?cellular cloningmolecular cloningreproductive cloning
molecular cloning
The technique that can be used for studying the gene for cystic fibrosis is molecular cloning.
Molecular cloning refers to the process of isolating and amplifying a specific DNA sequence of interest from a larger genome using recombinant DNA technology. In the case of studying the gene for cystic fibrosis, scientists can use molecular cloning to isolate the CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) gene and study it in detail to understand its structure and function.
Molecular cloning can be done using various methods, such as restriction enzyme digestion, PCR amplification, and gene synthesis. Once the CFTR gene has been isolated, it can be inserted into a vector, which is a carrier molecule that can be used to transfer the gene into a host cell. The host cell can then be used to produce large quantities of the CFTR protein for further analysis.
Molecular cloning has been essential in our understanding of the CFTR gene and the development of treatments for cystic fibrosis. By studying the gene at a molecular level, scientists have been able to identify mutations that lead to cystic fibrosis and develop targeted therapies that address the underlying genetic defect.
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