It is difficult for intrinsic (ab initio) gene-finding algorithms to accurately predict protein-coding genes in eukaryotic genomic DNA mostly because _____.A) exon/intron borders are hard to predictB) genes may be many kilobases in lengthC) promoters may contain single nucleotide polymorphisms (SNPs)D) coding regions may contain repetitive elements
A) exon/intron borders are hard to predict
A) Exon/intron borders are hard to predict.
In eukaryotic genomic DNA, predicting protein-coding genes can be challenging for intrinsic gene-finding algorithms. Exon/intron borders are hard to predict because they are not well-conserved and can vary in length and sequence. Additionally, alternative splicing events can result in multiple transcripts from the same gene, further complicating gene prediction. Therefore, it can be difficult for gene-finding algorithms to accurately identify the exact location and boundaries of protein-coding genes.
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