Best Practices for Creating FASTA Files: Proper Defining of Sequence Records with the Greater-Than Sign (>)

In a FASTA file with multiple sequences, _____.A) each sequence record should begin with the identifier of the sequenceB) the definition line of each sequence record should start with the number sign (#)C) the definition line of each sequence record should start with the greater-than character (>)D) the end of a sequence should be marked by an asterisk (*)

C) the definition line of each sequence record should start with the greater-than character (>)

C) The definition line of each sequence record should start with the greater-than character (>).

In a FASTA file with multiple sequences, each sequence record should begin with the greater-than symbol (>), which is known as the header or definition line. The header line contains important information about the sequence, such as its name or unique identifier. Following the header line, the actual sequence data is presented as a string of characters that represents the nucleotides or amino acids.

Option A is incorrect because the identifier should be part of the header line, not necessarily the first line of the record.

Option B is incorrect because the number sign (#) is not used to represent the start of a header line in FASTA format.

Option D is incorrect because an asterisk (*) is not used to mark the end of a sequence in FASTA format. Instead, the end of a sequence is indicated simply by the start of a new header line.

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