Advantages of High-Throughput DNA Sequencing for Efficient and Accurate Fragment Sequencing

High-throughput DNA sequencing a. is labor-intensive and expensive. b. uses unlabeled nucleotides. c. requires an RNA primer. d. is fully automated. e. can reliably sequence hundreds of DNA fragments at one time.

D

​The correct answer is e. High-throughput DNA sequencing can reliably sequence hundreds of DNA fragments at one time.

Option a is incorrect because high-throughput DNA sequencing is actually less labor-intensive and less expensive than traditional Sanger sequencing because it allows for the sequencing of many DNA fragments simultaneously.

Option b is incorrect because high-throughput DNA sequencing uses labeled nucleotides, which allows for the detection of incorporated nucleotides during the sequencing reaction.

Option c is incorrect because high-throughput DNA sequencing uses DNA primers, not RNA primers.

Option d is partially correct, as high-throughput DNA sequencing machines are automated to a large extent, but still require some manual input and attention from technicians. However, this is not the most important characteristic of this technology.

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