Which of the following contributes to regulation of the lac operon?a. cAMPb. allolactosec. the catabolite activated proteind. All of these contribute to lac operon regulation.
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D. All of these contribute to lac operon regulation.
Explanation:
The lac operon is a group of three genes in bacteria that encode for the enzymes involved in lactose metabolism. The expression of the genes in the lac operon is regulated by both negative and positive control mechanisms.
– cAMP: The regulation of the lac operon starts with the availability of glucose in the cell. When glucose is scarce, the levels of cyclic AMP (cAMP) increase in the cell. Cyclic AMP binds to the catabolite activator protein (CAP), which then binds to a specific site on the DNA to stimulate transcription of the lac operon.
– Allolactose: The lac operon is also regulated by the presence of lactose or its analog inducers. One of these inducers is allolactose, which is derived from lactose. Allolactose binds to the repressor protein, changing its shape so that it can no longer bind to the operator sequence on the DNA, which allows RNA polymerase to transcribe the genes.
– Catabolite Activated Protein: The catabolite activated protein (CAP) is a regulatory protein that can stimulate transcription when it is bound to the lac operon DNA. When glucose levels are low, cAMP-CAP binds to the promoter region of the operon and facilitates the binding of RNA polymerase to the DNA.
Therefore, all three of these mechanisms contribute to the regulation of the lac operon in bacteria.
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