Mohamed Abbas, a science teacher at Senioritis.io

Mohamed Abbas
August 27, 2023
Biochemistry

T7 Polymerase Transcription Efficiency

Which sequence characteristics influence the transcription efficiency of T7 polymerase? The transcription efficiency of T7 polymerase, or any RNA polymerase, is influenced by several sequence characteristics of...

Mohamed Abbas
August 27, 2023
Biochemistry

Producing Isotope-Labeled DNA Oligomers

How can I produce milligram quantities of an isotope-labeled DNA oligomer? Producing milligram quantities of isotope-labeled DNA oligomers involves several steps. Here is a detailed guide on...

Mohamed Abbas
August 27, 2023
Biochemistry

Chemistry of Whiteboard & Permanent Marker Odors

What are the olfactory chemicals in whiteboard/permanent markers and what do they bind to in the nose, lungs, and brain? Whiteboard and permanent markers contain various chemicals...

Mohamed Abbas
August 27, 2023
Biochemistry

siRNA and DNA Methylation in Mammalian Cells

Can siRNA induce DNA methylation in mammalian cells? No, small interfering RNA (siRNA) cannot directly induce DNA methylation in mammalian cells. siRNA acts as a mediator of...

Mohamed Abbas
August 27, 2023
Biochemistry

Thymine in DNA for Stability and Protection

Why is thymine rather than uracil used in DNA? Thymine (T) is used in DNA instead of uracil (U) for a specific reason related to the stability...

Mohamed Abbas
August 27, 2023
Biochemistry

Measuring Bacterial Alkaline Phosphatase Activity

How can I measure bacterial alkaline phosphatase activity? To measure bacterial alkaline phosphatase activity, you can use a colorimetric assay or a fluorescent assay. Here is a...

Mohamed Abbas
August 27, 2023
Biochemistry

The Implications of the Missing 2′-OH Group in DNA

What implications has the missing 2′-OH on the capability of DNA to form 3D structures? The missing 2′-OH (hydroxyl) group in DNA has significant implications for its...

Mohamed Abbas
August 27, 2023
Biochemistry

How pH and Salt Concentration Impact Protein Complexes

What effect has changing pH and salt concentration on protein complexes? Changing pH and salt concentration can significantly affect protein complexes. pH can influence protein structure and...