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  • Mohamed Abbas
  • July 4, 2023
  • Anatomy

The Role of Respiratory Bronchioles and Alveoli in Gas Exchange: A Comprehensive Overview.

respiratory bronchioles and alveoli The respiratory bronchioles and alveoli are two key structures of the respiratory system that play a crucial role in the process of gas...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

Understanding the Difference Between Liquid Tumors and Solid Tumors in Cancer: A Comprehensive Guide

Liquid tumor; Leukemia: bone marrow; Lyphoma: lymph nodes & tissueSolid tumor: bones & soft tissue A liquid tumor refers to a type of cancer that primarily affects...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

Detecting the EWS/FLI1 Fusion Transcript in Ewing’s Cancer: A Guide to using Reverse-Transcriptase PCR (RT-PCR) with Targeted Primers

EWS/ EWINGScancer/solid tumort(11:22)Reverse-Transcriptase PCR (RT-PCR)PCR primers designed to yield product only if translocation has occurred In the case of Ewing’s cancer, also known as Ewing’s sarcoma, a...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

Understanding Abnormal Lymphoblast Production in Bone Marrow

ALLt(9:22)FISH, CISH, RTPCRFound in the bone marrowhigh lymphoblast production leaves little or no normal cell production/population It seems you have provided some information about a specific condition...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

How Translocation Events Impact Gene Expression: Loss of Function in DNA Binding Proteins

PMLt(15:17)FISH, RTPCRRARA (15) + PML (17) genes both are DNA Binding proteinsWhen translocation = loss of function for both and affects gene expression due to lack of...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

Exploring the Role of CCND1 Overexpression in Mantle Cell Lymphoma: A Molecular Perspective for Targeted Therapies

MCLt(11:14)FISH, PCR, RTPCRMutations increase expression of CCND1 forcing cells from G1 to S phase. Affects B cells and develops in the mantle zone in the lymph node....
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

Unlocking the Potential: The Significance of the c-myc Gene in Gene Regulation and Cancer Development

BLt(8:14)FISH, CISHc-myc gene codes for transcription factor that controls transcription of other genes The c-myc gene is an important gene that codes for a transcription factor The...
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  • Mohamed Abbas
  • July 4, 2023
  • Pathology

A Step-by-Step Guide to Designing Primers for Translocation-Specific PCR: Understanding, Gene Sequences, Breakpoints, Designing Criteria, Validation

RMS/ARMScancer/solid tumort(2:13) or t(1:13)RTPCR, FISHPCR primers designed to yield product only if translocation has occurred To design primers for a translocation-specific PCR (Polymerase Chain Reaction), such as...
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