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  • Mohamed Abbas
  • June 20, 2023
  • Bioinformatics

Discover DNA Sequencing with Sanger Method: Principle, Techniques and Applications

Describe Sanger sequencing Template is denatured to form single strands, and extended with a polymerase in the presence of dideocynucleorides (ddNTPs) that cause chain termination Sanger sequencing,...
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  • Mohamed Abbas
  • June 20, 2023
  • Bioinformatics

Understanding Sanger Sequencing: Factors Affecting Read Lengths Explained

Typical read length for Sanger sequencing 800 base pairs Sanger sequencing is a widely used DNA sequencing technique that employs a chain termination method for determining the...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Expert Guide to Nortriptyline for Depression, Chronic Pain, Anxiety, and Migraines

nortriptyline tricyclic antidepressants (TCAs) Nortriptyline is a medication that belongs to the class of drugs known as tricyclic antidepressants (TCAs). It works by increasing the levels of...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Hydrochlorothiazide: A Guide to Use, Side Effects, Interactions, and Precautions

hydrochlorothiazide* thiazide diureticNa/Cl symporter, early distalhypokalemia can bring about metabolic alkalosispotentiates loops, hypokalemia can exacerbate digoxin toxicity, sulfa allergy Hydrochlorothiazide is a medication used to treat hypertension...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Furosemide: Mechanism of Action, Dosage, Side Effects, Precautions, Monitoring, and Patient Education

furosemide* loop diureticinhibition of the Na/K/2Cl symporter in ascending LOHincrease in sodium & H2O, more so than othershypocalcemia, hypokalemia can cause met. alkalosis and can exaggerate digoxin...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Understanding the Therapeutic Uses and Potential Benefits of Amiloride for Hypertension and Edema

amiloride potassium sparing diureticNa channel blocker, late distal tubule, indirectly reduces the secretion of K, used to prevent hypokalemiahyperkalemia w ARB’s/ACE’s or K supplements Amiloride is a...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Get Relief from Fluid Retention with the Potassium-Sparing Diuretic – Triamterene: Dosage, Side Effects, and Usage

triamterene potassium sparing diureticNa channel blocker, late distal tubule, indirectly reduces the secretion of K, used to prevent hypokalemiahyperkalemia w ARB’s/ACE’s or K supplements Triamterene is a...
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  • Mohamed Abbas
  • June 20, 2023
  • Medicine

Understanding Spironolactone: Uses, Mechanisms, and Side Effects

spironolactone potassium sparing diureticAldosterone antagonist, late distal tubule, blocks gene expression, prevents Na reab. and K secretionantiandrogenic effect, gynecomastia and impotencehyperkalemia w ARB’s/ACES/K supplements Spironolactone is a...
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