Mohamed Abbas, a science teacher at Senioritis.io

Mohamed Abbas
June 20, 2023
Biotechnology

An Overview of Polymerase Chain Reaction (PCR) – A Molecular Biology Technique for DNA Amplification

polymerase chain reaction (PCR) A technique for amplifying (copying) DNA in vitro by incubating with special primers, DNA polymerase molecules, and nucleotides. Polymerase Chain Reaction (PCR) is...

Mohamed Abbas
June 20, 2023
Biotechnology

Maximizing PCR Efficiency: The Significance of a Thermocycler in Molecular Biology Research

thermocycler Machine that rapidly changes DNA sample temperatures A thermocycler is an instrument used in molecular biology to amplify a specific DNA sequence through the polymerase chain...

Mohamed Abbas
June 20, 2023
Biotechnology

Discover the Benefits and Limitations of Taq Polymerase in PCR Reactions

Taq polymerase A heat-stable form of DNA polymerase extracted from bacteria that live in hot environments, such as hot springs, that is used during PCR technique Taq...

Mohamed Abbas
June 20, 2023
Biotechnology

Understanding the Basics of dNTPs: Essential Building Blocks for DNA Replication and Synthesis

dNTPs (deoxyribonucleoside triphosphates) spare nucleotides the polymerase uses to build the DNA sequence copies dNTPs (deoxyribonucleoside triphosphates) are building blocks of DNA (deoxyribonucleic acid) molecules. They are...

Mohamed Abbas
June 20, 2023
Biotechnology

Mastering the Denaturing Step in PCR: Crucial to Efficient Amplification of DNA Sequences

denaturing step of PCR The thermocycler heats the sample containing all the ingredients to a high temperature (94-95 C). This “denatures” the DNA strands, causing the two...

Mohamed Abbas
June 20, 2023
Molecular Biology

Understanding DNA Polymerases: Directionality, Specificity, and Replication.

What do DNA polymerases show? (Ex: 5 prime, 3 prime ends) Directionality DNA polymerases are enzymes that catalyze the formation of new DNA strands during DNA replication....

Mohamed Abbas
June 20, 2023
Molecular Biology

Understanding No De Novo Synthesis in Metabolic Pathways.

Definition of No de novo synthesis Nothing happens after incubation “No de novo synthesis” refers to a metabolic process in which a molecule or compound is not...

Mohamed Abbas
June 20, 2023
Molecular Biology

Optimizing DNA Replication: Understanding the Key Requirements for Active DNA Polymerase Function

What does DNA polymerase need to work? A primer; a partially double stranded piece of DNA DNA polymerase is an enzyme that plays a vital role in...