Identifying Point Mutation in DNA Sequence and its Impact on Amino Acid Sequence

DNA sequence (normal) – TAC GTA GTC ACC TAA TGG ATC Amino acid sequence – met his gln trp ile thr stop sequence 4 – TAC GTA GTC CCT AAT GGA TC… amino acid sequence – met his gln gly leu pro what type of mutation is this and why?

The mutation in the DNA sequence can be identified by comparing it to the normal DNA sequence

The mutation in the DNA sequence can be identified by comparing it to the normal DNA sequence. In this case, the normal DNA sequence is TAC GTA GTC ACC TAA TGG ATC, and the mutated sequence is TAC GTA GTC CCT AAT GGA TC.

By comparing the two sequences, we can identify a single base change in the DNA. The normal DNA sequence has “ACC” (adenine, cytosine, cytosine) at the 11th position, and the mutated sequence has “CCT” (cytosine, cytosine, thymine) at the same position.

This mutation is known as a point mutation or a substitution mutation. It occurs when a single nucleotide is replaced by another nucleotide in the DNA sequence. In this case, the base cytosine (C) has been replaced with thymine (T). As a result of this point mutation, the codon at the 11th position of the DNA sequence changes from “ACC” to “CCT”.

Consequently, this point mutation leads to a change in the encoded amino acid. The normal amino acid sequence corresponding to the DNA sequence is “met his gln trp ile thr stop”. However, due to the mutation, the amino acid sequence changes to “met his gln gly leu pro”.

Based on the change in the amino acid sequence, we can conclude that this mutation is a missense mutation. This is because the mutation results in the replacement of one amino acid (isoleucine) with another amino acid (glycine) in the protein sequence.

It is important to note that the functional impact of this mutation would depend on the specific protein that is encoded by this DNA sequence, as different amino acid changes can have varying effects on protein structure and function.

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