True or False Whole-genome shotgun sequencing cannot be used to sequence and assemble a large genome although many software tools are available for assembling shotgun reads into contigs.
F
False. Whole-genome shotgun sequencing can be used to sequence and assemble a large genome. Many software tools are available for assembling shotgun reads into contigs and scaffolds. However, it may be more challenging and require more computational resources to assemble a large genome compared to a smaller one. In addition, certain regions of the genome, such as highly repetitive or complex regions, may be more difficult to assemble accurately using only short-read shotgun sequencing. In these cases, alternative sequencing methods or additional long-read sequencing may be necessary to improve the accuracy of the assembly.
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