A260=1.4, A280=1.1 . protein contamination or Rna contam or useable RNA
Protein contamination . A260/280 = 1.3 <1.6 = protein contamination, 1.7-2.0 = good DNA, 1.9-2.0 = good RNA
The ratio A260/A280 is commonly used to determine the purity of RNA or DNA samples. While there is no definitive ratio for the purity of RNA or DNA samples, a ratio of around 2.0 for RNA samples is generally considered to be pure. However, in this case, the given A260/A280 ratio of 1.27 is lower than the expected ratio for RNA samples, indicating the presence of protein contamination.
Proteins are known to have an absorbance maximum around 280 nm, which is close to that of nucleic acids, and hence protein contamination can interfere with the determination of RNA or DNA concentrations. In this case, the A280 value of 1.1 is higher than expected for pure RNA samples, further supporting the presence of protein contamination.
Therefore, this sample is likely not suitable for RNA-based applications as its integrity and purity have been compromised by protein contamination. However, if the goal is to investigate protein content in the sample, then this RNA is potentially useable. It is recommended to perform additional analysis, such as gel electrophoresis, to confirm the presence and extent of protein contamination in the RNA sample.
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