What is the purpose of Lactophenol cotton blue (LPCB)?
It’s added to the specimen to visualize microscopic fungal morphology
Lactophenol cotton blue (LPCB) is a type of staining solution that is used in microbiology to observe and study fungi under a microscope. The solution contains lactophenol, which is a preservative that helps to maintain the fungal structures and cotton blue, which acts as a counterstain, allowing for better visualization of the fungal structures. The primary purpose of LPCB is to enhance the contrast and highlight the unique features of fungal cells, such as their hyphae, conidia, and spores. LPCB is particularly useful in identifying pathogenic fungi that may cause infections in humans and animals. It is often used in clinical laboratories to diagnose various fungal diseases, such as candidiasis, aspergillosis, and dermatophytosis.
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