Typical read length for Sanger sequencing
800 base pairs
Sanger sequencing is a widely used DNA sequencing technique that employs a chain termination method for determining the sequence of nucleotides in a DNA molecule. In Sanger sequencing, the length of the reads (the number of nucleotides that can be sequenced in a single run) depends on the length of the DNA fragment being sequenced and the quality of the sequencing reagents.
Typically, the read length for Sanger sequencing ranges from 500 to 1000 base pairs (bp) in length. However, the read length can vary depending on the type of sequencer used, the quality of the sequencing chemistry, and the template DNA being sequenced.
In modern Sanger sequencing technologies, the sequencing read length can be extended up to 1500 bp using the latest sequencing reagents and base-calling algorithms. However, the read length can also be shorter depending on the quality of the sequencing reagents, template DNA, and reaction conditions.
Therefore, the read length for Sanger sequencing can vary depending on a variety of factors, but typically it ranges from 500 to 1000 bp.
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