What is the consensus sequence from Part B that has coverage included on at least 3 of the 5 DNA fragments (3x coverage)?
The query coverage data indicate that part of the query sequence is not present in any mRNA transcript for insulin.The sequence is part of four distinct insulin mRNA transcripts.
To determine the consensus sequence from Part B that has coverage included on at least 3 of the 5 DNA fragments (3x coverage), we will start by aligning the five DNA sequences and looking for the most frequent nucleotide at each position. We can use bioinformatics tools such as a multiple sequence alignment software to do this.
Once we have the alignment, we can count the number of times each nucleotide appears at each position. We will consider only positions where at least 3 of the sequences have coverage.
For example, suppose we have the following alignment:
AT–CGA
ATT-AGA
ATTTCGA
ACCACGA
AT–CG-
The consensus sequence for this alignment would be:
ATTCCGA
We can see that at position 1, the most frequent nucleotide is “A”, which appears in all five sequences. At position 2, “T” is the most frequent nucleotide, appearing in four out of five sequences. At position 3, “T” is again the most frequent nucleotide, appearing in three out of five sequences. At position 4, “C” is the most frequent nucleotide, appearing in four out of five sequences. At position 5, “C” is again the most frequent nucleotide, appearing in three out of five sequences. Finally, at position 6, “G” is the most frequent nucleotide, appearing in four out of five sequences.
Therefore, the consensus sequence that has coverage included on at least 3 of the 5 DNA fragments (3x coverage) is “ATTCCGA”.
More Answers:
Improving the Resolution of Whole Genomic Sequencing: Next-Generation Technologies and AdvancementsRevolutionizing Genomics: Rapid, Cost-Effective Human Genome Sequencing with High-Throughput Technologies and Advanced Analysis Methods
Ensuring Genome Sequence Accuracy: Methods and Techniques for Optimal Results