Charguff’s rule
A=T ratio and C=G ratio
Chargaff’s rule states that in DNA, the amount of adenine (A) always equals the amount of thymine (T), and the amount of guanine (G) always equals the amount of cytosine (C). This means that the base pairs A-T and G-C are always held together in a double-stranded DNA molecule. This is because the A-T base pair has two hydrogen bonds holding it together, and the G-C base pair has three hydrogen bonds, making them a stable and complementary pairing. This rule played a crucial role in the discovery of the double helix structure of DNA by Watson and Crick, as it allowed them to deduce the complementary base pairing that is necessary for the two strands to twist around each other in a stable manner.
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