Isolate and differentiate Staphylococci bacterial species with MSA – a selective and differential agar

What types of organisms can grow in MSA?

salt-tolerant Gram-positive bacteria (Staphylococcus)

MSA stands for Mannitol Salt Agar, which is a selective and differential agar commonly used in microbiology labs to isolate and differentiate Staphylococci bacterial species. It contains high salt concentration that inhibits the growth of most bacterial species, except for staphylococci, which are salt-tolerant bacteria.

The following organisms can grow on MSA:
– Staphylococcus aureus (usually produces yellow colonies on MSA)
– Staphylococcus epidermidis (usually produces white colonies on MSA)
– Staphylococcus saprophyticus (usually produces yellow colonies on MSA)
– Staphylococcus haemolyticus (usually produces white colonies on MSA)
– Staphylococcus hominis (usually produces white colonies on MSA)
– Staphylococcus warneri (usually produces white colonies on MSA)

Therefore, MSA is a selective agar media that can be used to isolate and differentiate staphylococcal bacteria species from other bacterial species.

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