what does UV spectroscopy examine?
transitions b/t vibration energy levels
UV spectroscopy is a technique that examines the interaction of light with molecules in the ultraviolet (UV) region of the electromagnetic spectrum. It measures the absorption or transmission of ultraviolet light by a sample, which is typically a solution of molecules. UV spectroscopy is useful in identifying and quantifying the presence of organic molecules, such as nucleic acids and proteins, as well as inorganic compounds.
When a molecule absorbs light in the UV range, it promotes electrons from the ground state to the excited state. The energy difference between these two states is directly proportional to the wavelength of light absorbed. By measuring the intensity of the absorbed light, UV spectroscopy can provide information about the electronic structure of the molecule and its chemical composition. It is commonly used in analytical chemistry and biochemistry to detect, quantify, and characterize small molecules and biomolecules.
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