Purifying Protein 1: Achieving Protein Separation via Ion Exchange Chromatography.

Of the fractionation methods discussed in lectures, what one fractionation procedure could be used to purify protein 1 from a mixture of three proteins whose amino acid compositions are as follows?

25% Ala, 20% Gly, 20% Ser, 10% Ile, 10% Val, 5% Asn 5% Gln 5% Pro

One fractionation procedure that could be used to purify protein 1 from a mixture of three proteins with different amino acid compositions is ion exchange chromatography.

In ion exchange chromatography, the protein mixture is passed through a column containing an ion exchange resin that is either positively or negatively charged. Proteins with opposite charge to that of the resin will bind to the column while the proteins with similar charge will pass through. The bound proteins can then be eluted from the column by altering the pH or salt concentration to disrupt the ionic interactions.

In the case of the mixture of the three proteins, the amino acid composition of Protein 1 could potentially differ from the other two proteins in such a way that it would have a different charge. Therefore, by using ion exchange chromatography and choosing the appropriate ion exchange resin, Protein 1 could be selectively bound to the column while the other two proteins pass through. Afterward, Protein 1 can be eluted from the column, resulting in its purification.

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