Why should Haemophilus influenzae be grown in a candle jar?
Hameophilus influenzae is a bacteria that grows best in a higher concentration of CO2 and a lower concentration of oxygen. They are known as capnophiles. A candle jar consists of a jar with a tight-fitting lid that can accommodate the cultures and a candle. After the cultures are added to the jar, the candle is lit and the lid closed. As the candle burns, it consumes most of the oxygen present and releases CO2.
Haemophilus influenzae is a fastidious gram-negative bacterium that requires a complex growth environment to grow in laboratory conditions. The bacterium requires an atmosphere that is low in oxygen and rich in carbon dioxide to promote growth.
A candle jar is a special type of environment that facilitates the growth of Haemophilus influenzae by creating a low-oxygen and high-carbon dioxide environment. The candle jar contains a burning candle, which consumes oxygen, causing the remaining air to have low levels of oxygen, which helps the bacteria to grow. The combustion of the candle produces carbon dioxide, which further enriches the growth environment for the bacteria.
Therefore, using a candle jar is important when growing Haemophilus influenzae to provide an environment that meets its specific growth requirements. This culture technique increases the yield of viable bacteria, allowing scientists to study the organism in greater detail and provide accurate detection and diagnosis of the infection caused by this bacterium.
More Answers:
The Importance of Regulating Intra-Cellular pH Levels in Bacteria for Optimal Functioning of Electron Transport ChainMaintaining Optimal pH: The Key to Preserving Protein Structure and Function
Preventing Botulism in Canned Foods: Anaerobic Bacteria and Proper Canning Techniques