VDJ sequencing in mice, DNA or RNA?
VDJ sequencing in mice involves the analysis of DNA sequences. VDJ recombination is a process that occurs specifically in the DNA of immune cells, known as lymphocytes, in vertebrates. It is responsible for the production of diverse antigen receptor genes, which are essential for the immune system to recognize and respond to a wide range of pathogens.
The VDJ recombination process involves the rearrangement and joining of specific gene segments, namely Variable (V), Diversity (D), and Joining (J) segments, to generate a functional immune receptor gene. This process occurs in the DNA of developing immune cells in the bone marrow of mice and other mammals
The DNA sequence of the VDJ region can be analyzed using various sequencing technologies, such as Sanger sequencing or more recently, high-throughput sequencing methods like next-generation sequencing (NGS). These methods involve obtaining DNA samples from lymphocytes, isolating and amplifying the VDJ region of interest, and then sequencing the amplified DNA fragments. The resulting sequencing data can then be analyzed to determine the specific V, D, and J gene segments utilized, as well as any somatic mutations that may have occurred during the recombination process
Overall, VDJ sequencing in mice primarily involves the analysis of DNA sequences to study the diversity and functionality of antigen receptor genes, shedding light on the adaptive immune response in these animals
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